leucopus in the life cycles of both the tick vector and several pathogens ( Fig. While antibiotics are available for treatment, there are neither human vaccines nor broadly implementable tick control measures to prevent infections. Lyme disease and associated zoonoses continue to increase in incidence and to spread to previously unaffected areas in North America ( 4). musculus in this respect and make it a competent reservoir for a variety of pathogens are not known. leucopus that distinguish this species from M. leucopus in manifestations of and responses to infection ( 1, 3), but the genetic traits of P. musculus has been the animal model of choice for experimental studies of Lyme disease and other infections, the house mouse is not a natural reservoir for these infections. Peromyscines cluster with hamsters, voles, and wood rats rather than murids such as Mus musculus and Rattus norvegicus ( 2). The genus Peromyscus also includes the major hantavirus reservoir Peromyscus maniculatus, the North American deer mouse. The species is a major reservoir or carrier for several tick-borne diseases, including the bacterial infections Lyme disease, anaplasmosis, and Borrelia miyamotoi relapsing fever the malaria-like protozoan disease babesiosis and a fatal or disabling viral encephalitis ( 1). The white-footed mouse Peromyscus leucopus is a widely distributed, abundant rodent in eastern and central United States and adjoining regions of Canada and Mexico. Peromyscus leucopus is the major reservoir for several infectious diseases in North America The reference genome will allow for experiments aimed at elucidating the mechanisms by which this widely distributed rodent serves as natural reservoir for several infectious diseases of public health importance, potentially enabling intervention strategies. leucopus has a high level of segregating nucleotide variation, suggesting that natural resistance alleles to Crispr gene targeting constructs are likely segregating in wild populations. leucopus with Borreliella burgdorferi, a Lyme disease agent, shows that, unlike blood, the skin is actively responding to the infection after several weeks. The resulting assembly was 2.45 Gb in total length, with 24 chromosome-length scaffolds harboring 97% of predicted genes. To expand the knowledge base for this key species in life cycles of several pathogens, we assembled and scaffolded the P. The rodent Peromyscus leucopus is the natural reservoir of several tick-borne infections, including Lyme disease. R 2 as a function of distance for chromosome 10 SNPs. DEGs in the skin following infection with B. DEGs in the blood following infection with B. Gene Ontology categories showing change between tissues. Samples used in the tissue RNA-seq experiment. Summary of the RepeatMasker analysis of the P. Chromosome-sized scaffold lengths and names. Selected tracks from the Santa Cruz Browser genomehub interface for IL-6. SNPs and INDELs identified by aligning Illumina reads from the reference individual back to the assembly.įig. leucopus chromosomes reordered to reflect the scaffold numbers produced by 3d-dna.įig. Rat syntenic groups associated with the chromosome 16 and 21 fusion.įig. The Hi-C contact map for the region near the putative chromosome 16 and 21 fusion.įig. maniculatus linkage map (orange) and the P. Hi-C scaffolding results in chromosome-sized scaffolds that are considerably longer than the raw contigs.įig. Mummer dot plot for chromosome 3 assemblies.įig. Read span coverage as a function of read span size bins for Hi-C data summed over two Hi-C libraries.įig. Cumulative contig coverage (Mb) as a function of contig length for different PacBio/Illumina assembly strategies.įig. Supplementary material for this article is available at įig.
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